Puas yog dihydrocoumarin lom?

Dihydrocoumarin, tshuaj tsw qab, siv rau hauv cov khoom noj, kuj siv los ua coumarin hloov, siv los ua cov tshuaj pleev ib ce; Sib tov qab zib, txiv maj phaub, cinnamon tsw; Nws kuj siv los ua cov tshuaj luam yeeb.

Puas yog dihydrocoumarin lom?

Dihydrocoumarin tsis muaj tshuaj lom. Dihydrocoumarin yog ib yam khoom ntuj uas pom muaj nyob rau hauv cov vanilla daj rhinoceros. Nws yog npaj los ntawm hydrogenation ntawm coumarin nyob rau hauv lub xub ntiag ntawm nickel catalyst ntawm 160-200 ℃ thiab nyob rau hauv siab. Nws kuj tseem siv tau ua raw khoom, hydrolyzed nyob rau hauv alkaline aqueous kua los tsim o-hydroxyphenylpropionic acid, dehydration, kaw-loop tau txais.

Kev khaws cia

Kaw thiab tsaus ntuj, khaws cia rau qhov chaw txias thiab qhuav, qhov chaw hauv lub thoob me me li sai tau raws li kev tso cai kev nyab xeeb, thiab puv nrog kev tiv thaiv nitrogen. Khaws cia rau hauv lub tsev rau khoom txias, muaj cua nkag. Nyob deb ntawm hluav taws, dej. Yuav tsum khaws cia cais ntawm oxidizer, tsis txhob sib xyaw cia. Nruab nrog ntau yam thiab ntau npaum li cas ntawm cov khoom siv tua hluav taws.

Kev tshawb fawb hauv vitro

Kev sim tshuaj enzymatic hauv vitro, dihydrocoumarin ua rau muaj kev txwv tsis pub SIRT1 (IC50 ntawm 208μM) nyob ntawm qhov concentration. Kev txo qis hauv SIRT1 deacetylase kev ua haujlwm tau pom txawm tias ntawm micromolar koob tshuaj (85 ± 5.8 thiab 73 ± 13.7% kev ua haujlwm ntawm 1.6 μM thiab 8μM, feem). Microtubule SIRT2 deacetylase kuj tau raug txwv tsis pub ua zoo sib xws nyob ntawm qhov koob tshuaj (IC50 ntawm 295μM).

Tom qab 24 teev ntawm kev raug tshuaj, dihydrocoumarin (1-5mM) ua rau muaj kev phom sij ntau ntxiv rau cov kab mob TK6 cell nyob ntawm seb muaj pes tsawg koob tshuaj. Dihydrocoumarin (1-5mM) ua rau muaj kev tuag ntawm cov kab mob TK6 cell nyob ntawm seb muaj pes tsawg koob tshuaj ntawm lub sijhawm 6 teev. Kev siv 5mM ntawm dihydrocoumarin ua rau muaj kev tuag ntawm cov kab mob TK6 cell nyob ntawm seb muaj pes tsawg koob tshuaj. Tom qab 24 teev ntawm kev raug tshuaj, dihydrocoumarin (1-5mM) ua rau muaj kev acetylation ntawm p53 lysine 373 thiab 382 nyob ntawm seb muaj pes tsawg koob tshuaj hauv cov kab mob TK6 cell.